Anti-NDUFS8 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	4728
Protein Name / Synonyms	NDUFS8
Concentration (lot specific)	0.35 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CI-23k antibody, CI23KD antibody, TYKY antibody, NDUFS8 antibody, complex I-23kD antibody, complex I 23kDa subunit antibody, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial antibody, NADH-ubiquinone oxidoreductase 23 kDa subunit antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

24 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 210 of NDUFS8 (Uniprot ID# O00217)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

NDUFS8 antibody detects NDUFS8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
NDUFS8 antibody (107-11109) dilution: 1:500.

NDUFS8 antibody detects NDUFS8 protein at on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NDUFS8 antibody (107-11109) dilution: 1:500.

NDUFS8 antibody detects NDUFS8 protein at on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NDUFS8 antibody (107-11109) dilution: 1:500.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-11109 diluted at 1:1000

NDUFS8 antibody detects NDUFS8 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
12 % SDS-PAGE
NDUFS8 antibody (107-11109) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
NDUFS8 antibody
107-11109 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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