Anti-NDUFV2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	4729
Protein Name / Synonyms	NDUFV2
Concentration (lot specific)	0.44 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CI-24k antibody, NDUFV2 antibody, nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit antibody, complex I 24kDa subunit antibody, NADH dehydrogenase ubiquinone flavoprotein 2, mitochondrial antibody, complex I, mitochondrial respitory chain, 24 kD subunit antibody, NADH-ubiquinone oxidoreductase flavoprotein 2 antibody, NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial antibody, NADH-ubiquinone oxidoreductase 24 kDa subunit antibody, NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

27 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 249 of NDUFV2 (Uniprot ID# P19404)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

NDUFV2 antibody detects NDUFV2 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
NDUFV2 antibody (107-10331) dilution: 1:500

NDUFV2 antibody detects NDUFV2 protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
12 % SDS-PAGE
NDUFV2 antibody (107-10331) dilution: 1:500

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using NDUFV2(107-10331) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:A431
12% SDS PAGE
107-10331 diluted at 1:1500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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