NEFH Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P12036 |
| Gene Id | 4744 |
| Gene Symbols | NEFH |
| Protein Name / Synonyms | NEFH, CMT2CC, NFH, neurofilament heavy |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
105kDa/112kDa
Observed MW
200kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse brain, using NEFH antibody (144-08442) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (144-08442) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (144-08442) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (144-08442) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.