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Anti-NEFL Antibody
Catalog #: 144-00257
NEFL Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P07196 |
| Gene Id | 4747 |
| Gene Symbols | NEFL |
| Protein Name / Synonyms | NEFL, CMT1F, CMT2E, NF-L, NF68, NFL, PPP1R110, neurofilament light |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
61kDa
Observed MW
82kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-543 of human NEFL (NP_006149.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of MCF-7 cells, using NEFL antibody (144-00257) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using NEFL antibody (144-00257) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFL antibody (144-00257) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using NEFL antibody (144-00257) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using NEFL antibody (144-00257). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.