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Anti-NELFE Antibody
Catalog #: 144-07006
NELFE Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P18615 |
| Gene Id | 7936 |
| Gene Symbols | NELFE |
| Protein Name / Synonyms | NELFE, D6S45, NELF-E, RD, RDBP, RDP, negative elongation factor E |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
40kDa/43kDa
Observed MW
43kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NELFE antibody (144-07006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using NELFE antibody (144-07006).
Expiration:
12 months from the date of shipment when stored properly.