Anti-NeuroD1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13562
Gene Id	4760
Gene Symbols	NEUROD1
Protein Name / Synonyms	NeuroD1 (N-term)
Clonality	Polyclonal
Preparation	This NeuroD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human NeuroD1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NeuroD1 acts as a differentiation factor during neurogenesis. They are expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation. NeuroD1 is a basic helix-loop-helix (bHLH) protein contain 1 bHLH domain. NeuroD1 is a transcriptional activator, for efficient DNA binding it requires dimerization with another bHLH protein. It was reported that NeuroD1 involves heterodimerization with the ubiquitous bHLH protein E47, and regulates insulin gene expression by binding to a critical E-box motif on the insulin promoter. Defects in NEUROD1 causes maturity onset diabetes of the young type VI. MODY6 is a form of non-insulin-dependent diabetes mellitus (NIDDM) characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.

Specificity

Other Names

NEUROD1; BHLHA3; NEUROD; Neurogenic differentiation factor 1; Class A basic helix-loop-helix protein 3

NCBI Accession #

NP_002491.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39920

Recommended Dilutions

WB: 1:1000; IF: 1:100; IHC: 1:50-100

	Western blot analysis of hNeuroD1-Q30 (Cat# 102-14597) in HepG2 cell line lysates (35ug/lane). NEUROD1 (arrow) was detected using the purified Pab.
	Fluorescent confocal image of HepG2 cell stained with hNeuroD1-Q30(Cat# 102-14597). HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hNeuroD1-Q30 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).hNeuroD1-Q30 immunoreactivity is localized to vesicles significantly.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	ES cells were transiently transfected with flag-tagged mouse NeuroD1 (tagged on N-term).Fixed 24h post transfection.Stained for flag tag (red) to check that some cells express protein. Most protein was in nucleus but some was cytoplasmic. Stained with NeuroD1 N-term antibodies at 1:100. NeuroD1 N-term antibody showed strong and clear staining with similar pattern to the flag staining.(Supplied by Sally Lowell,Edinburgh University)

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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