Anti-NFATC3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q12968
Gene Id	4775
Gene Symbols	NFATC3
Protein Name / Synonyms	NFATC3, NFAT4, NFATX, nuclear factor of activated T-cells 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

77kDa/80kDa/112kDa/115kDa

Observed MW

150kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NFATC3 antibody (144-06666) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - NFATC3 antibody (144-06666)

Immunofluorescence analysis of HeLa cells using NFATC3 antibody (144-06666). Blue: DAPI for nuclear staining.
Immunofluorescence - NFATC3 antibody (144-06666)

Expiration:

12 months from the date of shipment when stored properly.

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