Anti-NFE2L1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q14494
Gene Id	4779
Gene Symbols	NFE2L1
Protein Name / Synonyms	NFE2L1, LCR-F1, NRF1, TCF11, nuclear factor, erythroid 2 like 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.01% thimerosal, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for 'nuclear respiratory factor 1' which has an official symbol of NRF1.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

81kDa/84kDa

Observed MW

64kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 515-772 of human NFE2L1 (NP_003195.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NFE2L1 antibody (144-60777) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - NFE2L1 Polyclonal Antibody (144-60777)

Expiration:

12 months from the date of shipment when stored properly.

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