Rabbit Anti-Human NFIA (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q12857 |
| Gene Id | 4774 |
| Gene Symbols | NFIA |
| Protein Name / Synonyms | NFIA (C-term) |
| Clonality | Polyclonal |
| Preparation | This NFIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-498 amino acids from the C-terminal region of human NFIA. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken
Other Names
NFIA; KIAA1439; Nuclear factor 1 A-type; CCAAT-box-binding transcription factor; Nuclear factor I/A; TGGCA-binding protein
NCBI Accession #
NP_001128145.1;NP_001138983.1;NP_001138984.1;NP_005586.1
Other Accession #
P09414; Q02780; P17923; NP_001138983.1; NP_001138984.1; NP_005586.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55944
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | NFIA Antibody (C-term) (Cat# 102-12328) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NFIA antibody detected the NFIA protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with NFIA Antibody (C-term)(Cat# 102-12328).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFIA immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.