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Anti-NFIC Antibody
Catalog #: 144-60904
NFIC Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P08651 |
| Gene Id | 4782 |
| Gene Symbols | NFIC |
| Protein Name / Synonyms | NFIC, CTF, CTF5, NF-I, NFI, nuclear factor I C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
45kDa/47kDa/48kDa/54kDa/55kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NFIC (NP_005588.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NFIC antibody (144-60904) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.