3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-NFKB1 Antibody
Catalog #: 144-11160
NFKB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P19838 |
| Gene Id | 4790 |
| Gene Symbols | NFKB1 |
| Protein Name / Synonyms | NFKB1, CVID12, EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkappaB, p105, p50, nuclear factor kappa B subunit 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 1.37 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer, Inflammation, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:20 - 1:50
Format
Calculated MW
85kDa/105kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NFKB1 antibody (144-11160) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using NFKB1 antibody (144-11160) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB1 antibody (144-11160) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using NFKB1 antibody (144-11160).
Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug NFKB1 antibody (144-11160). Western blot was performed from the immunoprecipitate using NFKB1 antibody (144-11160) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.