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Anti-NFKBIB Antibody
Catalog #: 144-05777
NFKBIB Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15653 |
| Gene Id | 4793 |
| Gene Symbols | NFKBIB |
| Protein Name / Synonyms | NFKBIB, IKBB, TRIP9, NFKB inhibitor beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
35kDa/37kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NFKBIB antibody (144-05777) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using NFKBIB antibody (144-05777) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using NFKBIB antibody (144-05777) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using NFKBIB antibody (144-05777) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using NFKBIB antibody (144-05777). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.