Anti-NFKBp65 (C-term S536) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q04206
Gene Id	5970
Gene Symbols	RELA
Protein Name / Synonyms	NFKBp65 (C-term S536)
Clonality	Polyclonal
Preparation	This NFKBp65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-539 amino acids from the C-terminal region of human NFKBp65.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Western Blotting
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Specificity

Predicted Reactivity

Mouse, Pig

Other Names

RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

NCBI Accession #

NP_001138610.1;NP_001230913.1;NP_068810.3

Other Accession #

Q04207; Q6QN13; P49895; NP_001138610.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60219

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of U251 cell stained with NFKBp65 Antibody(C-termS536)(Cat# 102-13731).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFKBp65 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).NFKBp65 immunoreactivity is localized to Cytoplasm significantly.

NFKBp65 Antibody (pS536) (Cat# 102-13731) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NFKBp65 antibody detected the NFKBp65 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products