Anti-NFS1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y697
Gene Id	9054
Gene Symbols	NFS1
Protein Name / Synonyms	NFS1, HUSSY-08, IscS, NIFS, NFS1, cysteine desulfurase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

44kDa/50kDa

Observed MW

45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NFS1 antibody (144-06668) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - NFS1 Polyclonal Antibody (144-06668)

Immunofluorescence analysis of MCF7 cells using NFS1 antibody (144-06668). Blue: DAPI for nuclear staining.
Immunofluorescence - NFS1 Polyclonal Antibody (144-06668)

Expiration:

12 months from the date of shipment when stored properly.

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