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Anti-NGEF (N-term) Antibody
Catalog #: 102-24715
Rabbit Anti-NGEF (N-term) Antibody, 200 µl
Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | Q8N5V2 |
| Gene Id | 25791 |
| Gene Symbols | NGEF |
| Protein Name / Synonyms | Ephexin-1, Eph-interacting exchange protein, Neuronal guanine nucleotide exchange factor, NGEF |
| Clonality | Polyclonal |
| Clone | RB53236 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
Other Information
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
82496
Antigen Source
Human
Images
WB (1:1000-1:2000)
All lanes : Anti-NGEF Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.