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Anti-NIN Antibody
Catalog #: 144-08215
NIN Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8N4C6 |
| Gene Id | 51199 |
| Gene Symbols | NIN |
| Protein Name / Synonyms | NIN, SCKL7, ninein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
147kDa/160kDa/236-248kDa
Observed MW
200kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using NIN antibody (144-08215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using NIN antibody (144-08215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody (144-08215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody (144-08215) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NIN antibody (144-08215) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.