Anti-NKX1-1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15270
Gene Symbols	NKX1-1
Protein Name / Synonyms	NKX1-1 (Center)
Clonality	Polyclonal
Preparation	This NKX1-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the Central region of human NKX1-1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The function of this protein remains unknown.

Specificity

Other Names

NKX1-1; HPX153; NK1 transcription factor-related protein 1; Homeobox protein 153; Homeobox protein SAX-2; NKX-1.1

Other Accession #

XP_931434.4

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

40659

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

NKX1-1 Antibody (Center) (Cat# 102-11309) western blot analysis in SK-N-MC cell line lysates (35ug/lane).This demonstrates the NKX1-1 antibody detected the NKX1-1 protein (arrow).

Fluorescent confocal image of Hela cell stained with NKX1-1 Antibody (Center)(Cat# 102-11309).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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