Anti-NLK Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	51701
Protein Name / Synonyms	NLK
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp761G1211 antibody, FLJ21033 antibody, NLK antibody, serine/threonine-protein kinase NLK antibody, nemo-like kinase antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

58 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 234 and 527 of NLK (Uniprot ID# Q9UBE8)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1-100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10717 diluted at 1:1000

NLK antibody [N3C3] detects NLK protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
NLK antibody [N3C3] (107-10717) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NLK(107-10717) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
NLK antibody
107-10717 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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