Anti-NME1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P15531
Gene Id	4830
Gene Symbols	NME1
Protein Name / Synonyms	NME1, AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1, nucleoside diphosphate kinase A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

17kDa/19kDa/30kDa

Observed MW

21kDa

Immunogen

A synthetic peptide of human NME1

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NME1 antibody (144-00259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - NME1 Polyclonal Antibody (144-00259)

Immunohistochemistry of paraffin-embedded rat heart using NME1 Antibody (144-00259) at dilution of 1:200 (40x lens).
Immunohistochemistry - NME1 Polyclonal Antibody (144-00259)

Immunohistochemistry of paraffin-embedded mouse brain using NME1 Antibody (144-00259) at dilution of 1:200 (40x lens).
Immunohistochemistry - NME1 Polyclonal Antibody (144-00259)

Immunofluorescence analysis of A549 cells using NME1 antibody (144-00259). Blue: DAPI for nuclear staining.
Immunofluorescence - NME1 Polyclonal Antibody (144-00259)

Expiration:

12 months from the date of shipment when stored properly.

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