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Anti-NME2 Antibody
Catalog #: 144-07443
NME2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P22392 |
| Gene Id | 4831 |
| Gene Symbols | NME2 |
| Protein Name / Synonyms | NME2, NDKB, NDPK-B, NDPKB, NM23-H2, NM23B, PUF, nucleoside diphosphate kinase B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
17kDa/30kDa
Observed MW
17kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human NME2 (NP_001018148.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NME2 antibody (144-07443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human kidney using NME2 antibody (144-07443) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using NME2 antibody (144-07443) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using NME2 antibody (144-07443). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.