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Anti-Nono Antibody
Catalog #: 144-05282
Nono Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15233 |
| Gene Id | 4841 |
| Gene Symbols | NONO |
| Protein Name / Synonyms | NONO, MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114, non-POU domain containing, octamer-binding |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
43kDa/54kDa
Observed MW
58kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Nono antibody (144-05282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Nono antibody (144-05282).
Expiration:
12 months from the date of shipment when stored properly.