NOS3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P29474 |
| Gene Id | 4846 |
| Gene Symbols | NOS3 |
| Protein Name / Synonyms | NOS3, ECNOS, eNOS, nitric oxide synthase 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
67kDa/68kDa/133kDa
Observed MW
140kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NOS3 (NP_000594.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NOS3 antibody (144-01548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.