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ISO 13485:2016 cGMP
Anti-NOX5 Antibody
Catalog #: 144-07136
NOX5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q96PH1 |
| Gene Id | 79400 |
| Gene Symbols | NOX5 |
| Protein Name / Synonyms | NOX5, NADPH oxidase 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis, Oxidative Stress |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
64kDa/82kDa/83kDa/84kDa/86kDa
Observed MW
69kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 661-765 of human NOX5 (NP_078781.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of 22Rv1 cells, using NOX5 antibody (144-07136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.