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ISO 13485:2016 cGMP

Anti-NPRL2 Antibody

Catalog #: 144-05859

NPRL2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q8WTW4
Gene Id
10641
Gene Symbols
NPRL2
Protein Name / Synonyms
NPRL2, FFEVF2, NPR2, NPR2L, TUSC4, NPR2-like, GATOR1 complex subunit
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCancer, Autophagy
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
23kDa/43kDa
Observed MW
40kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NPRL2 (NP_006536.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NPRL2 antibody (144-05859) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NPRL2 antibody (144-05859)

Immunofluorescence analysis of HeLa cells using NPRL2 antibody (144-05859). Blue: DAPI for nuclear staining.
Immunofluorescence - NPRL2 antibody (144-05859)

Expiration:
12 months from the date of shipment when stored properly.