RayBiotech

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ISO 13485:2016 cGMP

Anti-NQO2 Antibody

Catalog #: 144-11742

NQO2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P16083
Gene Id
4835
Gene Symbols
NQO2
Protein Name / Synonyms
NQO2, DHQV, DIA6, NMOR2, QR2, N-ribosyldihydronicotinamide:quinone reductase 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
25kDa
Observed MW
25kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-231 of human NQO2 (NP_000895.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NQO2 antibody (144-011742) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NQO2 antibody (144-011742)

Expiration:
12 months from the date of shipment when stored properly.