Anti-NR1H3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13133
Gene Id	10062
Gene Symbols	NR1H3
Protein Name / Synonyms	NR1H3, LXR-a, LXRA, RLD-1, oxysterols receptor LXR-alpha
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

43kDa/45kDa/50kDa

Observed MW

45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-330 of human NR1H3 (NP_005684.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NR1H3 antibody (144-02141) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - NR1H3 antibody (144-02141)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products