3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-NRBF2 Antibody
Catalog #: 144-06462
NRBF2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96F24 |
| Gene Id | 29982 |
| Gene Symbols | NRBF2 |
| Protein Name / Synonyms | NRBF2, COPR, COPR1, COPR2, NRBF-2, nuclear receptor-binding factor 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
26kDa/31kDa/32kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 10-220 of human NRBF2 (NP_110386.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NRBF2 antibody (144-06462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using NRBF2 antibody (144-06462). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.