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Anti-NSE Antibody, Sodium Azide Free
Catalog #: 188-11252-SAF
Mouse anti-Human/Mouse/Rat NSE Antibody / Neuron Specific Enolase [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P09104 |
| Gene Id | 2026 |
| Gene Symbols | ENO2 |
| Protein Name / Synonyms | Gamma-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Enolase 2) (Neural enolase) (Neuron-specific enolase) (NSE) |
| Immunogen | Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody. |
| Isotype | IgG2b |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Storage
Store the Neuron Specific Enolase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.