Anti-NSFL1C Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UNZ2
Gene Id	55968
Gene Symbols	NSFL1C
Protein Name / Synonyms	NSFL1C, P47, UBX1, UBXD10, UBXN2C, dJ776F14.1, NSFL1 cofactor
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

28kDa/37kDa/40kDa

Observed MW

41kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NSFL1C antibody (144-06677) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - NSFL1C Polyclonal Antibody (144-06677)

Immunofluorescence analysis of HeLa cells using NSFL1C antibody (144-06677). Blue: DAPI for nuclear staining.
Immunofluorescence - NSFL1C Polyclonal Antibody (144-06677)

Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (144-06677). Western blot was performed from the immunoprecipitate using NSFL1C antibody (144-06677) at a dilition of 1:1000.
Immunoprecipitation - NSFL1C Polyclonal Antibody (144-06677)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products