Anti-NUDT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P36639
Gene Id	4521
Gene Symbols	NUDT1
Protein Name / Synonyms	NUDT1, MTH1, nudix hydrolase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50

Format

Calculated MW

17kDa/19kDa/20kDa/22kDa

Observed MW

18kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NUDT1 antibody (144-60285) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)

Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 antibody (144-60285) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)

Immunohistochemistry of paraffin-embedded human gastric cancer using NUDT1 antibody (144-60285) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug NUDT1 antibody (144-60285). Western blot was performed from the immunoprecipitate using NUDT1 antibody (144-60285) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)

Expiration:

12 months from the date of shipment when stored properly.

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