RayBiotech

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Anti-NUDT15 Antibody

Catalog #: 144-08368

NUDT15 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9NV35
Gene Id
55270
Gene Symbols
NUDT15
Protein Name / Synonyms
NUDT15, MTH2, NUDT15D, nudix hydrolase 15
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
18kDa
Observed MW
19kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human NUDT15 (NP_060753.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NUDT15 antibody (144-08368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NUDT15 antibody (144-08368)

Immunohistochemistry of paraffin-embedded human liver using NUDT15 antibody (144-08368) at dilution of 1:100 (40x lens).
Immunohistochemistry - NUDT15 antibody (144-08368)

Immunohistochemistry of paraffin-embedded human stomach using NUDT15 antibody (144-08368) at dilution of 1:100 (40x lens).
Immunohistochemistry - NUDT15 antibody (144-08368)

Expiration:
12 months from the date of shipment when stored properly.