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Anti-NuMA Antibody, Sodium Azide Free
Catalog #: 188-10613-SAF
Mouse anti-Human NuMA Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | Q14980 |
| Gene Id | 4926 |
| Gene Symbols | NUMA1,NMP22,NUMA |
| Protein Name / Synonyms | Nuclear mitotic apparatus protein 1 (NuMA protein) (Nuclear matrix protein-22) (NMP-22) (SP-H antigen) |
| Immunogen | Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody. |
| Isotype | Kappa, IgM |
| Purity | PEG precipitation |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Western blot 1-2ug/ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1) |
| Domain / Region / Terminus | Nuclear |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
Storage
Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.