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Anti-NUMA1 Antibody
Catalog #: 144-00527
NUMA1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14980 |
| Gene Id | 4926 |
| Gene Symbols | NUMA1 |
| Protein Name / Synonyms | NUMA1, NMP-22, NUMA, nuclear mitotic apparatus protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
109kDa/200kDa/201kDa/236kDa/238kDa
Observed MW
238kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NUMA1 antibody (144-00527) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using NUMA1 antibody (144-00527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using NUMA1 antibody (144-00527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using NUMA1 antibody (144-00527) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NUMA1 antibody (144-00527) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.