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Anti-NUMB Antibody
Catalog #: 144-09352
NUMB Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49757 |
| Gene Id | 8650 |
| Gene Symbols | NUMB |
| Protein Name / Synonyms | NUMB, C14orf41, S171, c14_5527, protein numb homolog |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
37kDa/48-70kDa
Observed MW
71kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 521-640 of human NUMB (NP_003735.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NUMB antibody (144-09352) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.