3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-OCLN Antibody
Catalog #: 144-02601
OCLN Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16625 |
| Gene Id | 100506658 |
| Gene Symbols | OCLN |
| Protein Name / Synonyms | OCLN, BLCPMG, PPP1R115, PTORCH1, occludin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
Dilutions
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
8kDa/23kDa/31kDa/52kDa/54kDa/59kDa
Observed MW
59kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human OCLN (NP_002529.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using OCLN antibody (144-02601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat pancreas using OCLN antibody (144-02601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse ovary using OCLN antibody (144-02601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using OCLN antibody (144-02601) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using OCLN antibody (144-02601) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using OCLN antibody (144-02601) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using OCLN antibody (144-02601) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using OCLN antibody (144-02601) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.