Anti-OGN Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P20774
Gene Id	4969
Gene Symbols	OGN
Protein Name / Synonyms	OGN, OG, OIF, SLRR3A, mimecan
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200

Format

Calculated MW

33kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-298 of human OGN (NP_148935.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using OGN antibody (144-06679) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - OGN antibody (144-06679)

Immunohistochemistry of paraffin-embedded rat brain using OGN antibody (144-06679) at dilution of 1:100 (40x lens).
Immunohistochemistry - OGN antibody (144-06679)

Immunohistochemistry of paraffin-embedded human stomach using OGN antibody (144-06679) at dilution of 1:100 (40x lens).
Immunohistochemistry - OGN antibody (144-06679)

Expiration:

12 months from the date of shipment when stored properly.

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