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Anti-OPLAH Antibody
Catalog #: 144-12032
OPLAH Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14841 |
| Gene Id | 26873 |
| Gene Symbols | OPLAH |
| Protein Name / Synonyms | OPLAH, 5-Opase, OPLA, OPLAHD, 5-oxoprolinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
137kDa
Observed MW
134kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_060040.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using OPLAH antibody (144-012032) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.