Anti-OXA1L Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15070
Gene Id	5018
Gene Symbols	OXA1L
Protein Name / Synonyms	OXA1L, OXA1, mitochondrial inner membrane protein OXA1L
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an evolutionarily conserved protein that is localized to the inner mitochondrial membrane. The encoded protein is essential for the translocation of the N-terminal tail of subunit 2 of cytochrome c oxidase, and is involved in the assembly of the cytochrome c oxidase and ATPase complexes of the mitochondrial respiratory chain.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

28kDa/46kDa/48kDa

Observed MW

42kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 396-495 of human OXA1L (NP_005006.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using OXA1L antibody (144-06300) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - OXA1L antibody (144-06300)

Immunohistochemistry of paraffin-embedded rat liver using OXA1L antibody (144-06300) at dilution of 1:100 (40x lens).
Immunohistochemistry - OXA1L antibody (144-06300)

Immunofluorescence analysis of NIH/3T3 cells using OXA1L antibody (144-06300) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - OXA1L antibody (144-06300)

Expiration:

12 months from the date of shipment when stored properly.

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