Anti-OXCT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P55809
Gene Id	5019
Gene Symbols	OXCT1
Protein Name / Synonyms	OXCT1, OXCT, SCOT, 3-oxoacid CoA-transferase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

13kDa/56kDa

Observed MW

56kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using OXCT1 antibody (144-08139) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)

Western blot analysis of extracts from normal (control) and OXCT1 knockout (KO) HeLa cells, using OXCT1 antibody (144-08139) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)

Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody (144-08139) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)

Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody (144-08139) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)

Expiration:

12 months from the date of shipment when stored properly.

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