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ISO 13485:2016 cGMP

Anti-OXT Antibody

Catalog #: 144-60983

OXT Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P01178
Gene Id
5020
Gene Symbols
OXT
Protein Name / Synonyms
OXT, OT, OT-NPI, OXT-NPI, oxytocin-neurophysin 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.01% thimerosal, 50% glycerol, pH 7.3
Recommended ApplicationsWestern Blotting
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
12kDa
Observed MW
13kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human OXT (NP_000906.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using OXT antibody (144-60983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - OXT antibody (144-60983)

Expiration:
12 months from the date of shipment when stored properly.