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Anti-p21 Antibody
Catalog #: 130-10051
Rabbit Anti-Human p21 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat p21 has not yet been tested. |
| Accession Number | P38936 |
| Gene Id | 1026 |
| Gene Symbols | CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human p21. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | SEPAGDVRQNPCGSKAC |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000(at least detecting 12.5ng/ml)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The encode protein of p21 is the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. p21 binds to and inhibits cyclindependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions of p21 in the nuclear localization and assembly of cyclin D-CDK4 complex, promotes its kinase activity towards RB1. At higher stoichiometric ratios, p21 inhibits the kinase activity of the cyclin D-CDK4 complex.
Typical Data
Storage/Stability
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Expiration
12 months from the date of shipment when stored properly.
References
- Harper J.W., Adami G.R., Wei N., Keyomarsi K., Elledge S.J. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75:805-816(1993)
- Jaffer ZM, et al.. (2002) p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol. 34(7):713-7.