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Anti-p21 Antibody Cocktail
Catalog #: 188-10301-IHC
Mouse anti-Human p21 Antibody Cocktail [IHC only]
Product Description
Specifications
| Size | 7 mL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Does not react with mouse and rat. Other species not tested. |
| Host Species | Mouse |
| Accession Number | P38936 |
| Gene Id | 1026 |
| Gene Symbols | CDKN1A,CDKN1,CAP20,CIP1,MDA6,PIC1,SDI1,WAF1 |
| Protein Name / Synonyms | Cyclin-dependent kinase inhibitor 1 (CDK-interacting protein 1) (Melanoma differentiation-associated protein 6) (MDA-6) (p21) |
| Immunogen | Recombinant full-length human protein (CIP1/823 and DCS-60.2) was used as the immunogen for the p21 antibody cocktail. |
| Isotype | IgG |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide, *For IHC use only* |
| Recommended Applications | Prediluted format : incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Nuclear |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This mAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Storage
Store the p21 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.