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Anti-p27 Antibody Cocktail, Sodium Azide Free
Catalog #: 188-10304-SAF
Mouse anti-Human/Monkey/Mouse/Rat p27 Antibody Cocktail [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | P46527 |
| Gene Id | 1027 |
| Gene Symbols | CDKN1B,KIP1 |
| Protein Name / Synonyms | Cyclin-dependent kinase inhibitor 1B (Cyclin-dependent kinase inhibitor p27) (p27Kip1) |
| Immunogen | Mouse recombinant protein (DCS-72.F6) and recombinant human protein (KIP1/769) were used as the immunogen for the p27 antibody cocktail. |
| Isotype | IgG |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml Western blot: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Nuclear |
| Research Area | Cardiovascular Disease, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
Storage
Store the p27 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.