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Anti-P53 (176-200aa) Antibody
Catalog #: 130-11078
Rabbit Anti-Human P53 (176-200aa) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04637 |
| Gene Id | 7157 |
| Gene Symbols | TP53, P53 |
| Protein Name / Synonyms | Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53) |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen P53 (176-200aa): CPHHERCSDSDGLAPPQHLIRVEGN. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Immunogen | Synthetic immunogen P53 (176-200aa): CPHHERCSDSDGLAPPQHLIRVEGN |
| Recommended Applications | ELISA (recommended work dilution= 1:5, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Apoptosis, Cancer, p53 Signaling, Autophagy, DNA Damage |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation. The name p53 is in reference to its apparent molecular mass: It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. But, based on calculations from its amino acid residues, p53's mass is actually only 43.7 kDa. This difference is due to the high number of proline residues in the protein, which slows its migration on SDS-PAGE, thus making it appear heavier than it actually is. This effect is observed with p53 from a variety of species, including humans, rodents, frogs, and fish.
Indirect Fluorescent Antibody (IFA) Analysis
Alexa Fluor 488 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: nucleus, cytoplasm
CY3 goat anti-rabbit (1:200)
293T cells
Subcellular Location: nucleus, cytoplasm
Immunohistochemistry (IHC) Analysis
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.