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Anti-p53 / TP53 Antibody, Sodium Azide Free
Catalog #: 188-11265-SAF
Mouse anti-Human p53 Antibody / TP53 [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P04637 |
| Gene Id | 7157 |
| Gene Symbols | TP53,P53 |
| Protein Name / Synonyms | Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53) |
| Immunogen | Recombinant human protein was used as the immunogen for the TP53 antibody. |
| Isotype | IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 1-2ug/ml Western blot: 0.5-1ug/ml Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) |
| Domain / Region / Terminus | Nuclear |
| Research Area | Apoptosis, Cancer, p53 Signaling, Autophagy, DNA Damage |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Storage
Store the TP53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.