RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-PAH Antibody

Catalog #: 144-01559

PAH Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P00439
Gene Id
5053
Gene Symbols
PAH
Protein Name / Synonyms
PAH, PH, PKU, PKU1, phenylalanine hydroxylase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
51kDa
Observed MW
55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PAH (NP_000268.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PAH antibody (144-01559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PAH Polyclonal Antibody (144-01559)

Expiration:
12 months from the date of shipment when stored properly.