Anti-PAK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13177
Gene Id	5062
Gene Symbols	PAK2
Protein Name / Synonyms	PAK2, PAK65, PAKgamma, p21 (RAC1) activated kinase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

58kDa

Observed MW

58kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PAK2 (NP_002568.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PAK2 antibody (144-07333) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - PAK2 Polyclonal Antibody (144-07333)

Immunohistochemistry of paraffin-embedded mouse kidney using PAK2 antibody (144-07333) at dilution of 1:100 (40x lens).
Immunohistochemistry - PAK2 Polyclonal Antibody (144-07333)

Immunofluorescence analysis of A549 cells using PAK2 antibody (144-07333). Blue: DAPI for nuclear staining.
Immunofluorescence - PAK2 Polyclonal Antibody (144-07333)

Expiration:

12 months from the date of shipment when stored properly.

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