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Anti-Pan Cytokeratin Antibody Cocktail, Sodium Azide Free
Catalog #: 188-10215-SAF
Mouse anti-Human/Monkey/Cow/Dog/Rabbit/Mouse/Rat/Chicken Pan Cytokeratin Antibody Cocktail [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P04264,P12035,P13647,P02538,P05787,P13645,P02533,P19012,P08779,P08727 |
| Gene Id | 3880|3856|3850|3853|3858|3848|3866|3861|3852|3868|3851 |
| Gene Symbols | KRT8,KRT5,KRT6A,KRT3,KRT10,KRT1,KRT15,KRT14,KRT16,K6A,KRT6D,CYK8,KPP,KRTA,KRTB,KRT16A |
| Protein Name / Synonyms | Keratin, type II cytoskeletal 1 (67 kDa cytokeratin) (Cytokeratin-1) (CK-1) (Hair alpha protein) (Keratin-1) (K1) (Type-II keratin Kb1),Keratin, type II cytoskeletal 3 (65 kDa cytokeratin) (Cytokeratin-3) (CK-3) (Keratin-3) (K3) (Type-II keratin Kb3),Keratin, type II cytoskeletal 3 (65 kDa cytokeratin) (Cytokeratin-3) (CK-3) (Keratin-3) (K3) (Type-II keratin Kb3),Keratin, type II cytoskeletal 5 (58 kDa cytokeratin) (Cytokeratin-5) (CK-5) (Keratin-5) (K5) (Type-II keratin Kb5),Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK-6A) (Cytokeratin-6D) (CK-6D) (Keratin-6A) (K6A) (Type-II keratin Kb6) (allergen Hom s 5),Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8),Keratin, type I cytoskeletal 10 (Cytokeratin-10) (CK-10) (Keratin-10) (K10),Keratin, type I cytoskeletal 14 (Cytokeratin-14) (CK-14) (Keratin-14) (K14),Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15),Keratin, type I cytoskeletal 16 (Cytokeratin-16) (CK-16) (Keratin-16) (K16) |
| Immunogen | Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody. |
| Isotype | IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | FACS: 0.5-1ug/10e6 cells IF: 1-2ug/ml Western Blotting: 0.5-1ug/ml,for 2 hours at RT IHC (FFPE): 0.5-1ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of keratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Storage
Store the pan Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.