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Anti-PARG Antibody
Catalog #: 144-10577
PARG Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q86W56 |
| Gene Id | 8505 |
| Gene Symbols | PARG |
| Protein Name / Synonyms | PARG, PARG99, poly(ADP-ribose) glycohydrolase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:2000
Format
Calculated MW
54kDa/60kDa/99kDa/102kDa/111kDa
Observed MW
111kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 777-976 of human PARG (NP_003622.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PARG antibody (144-10577) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Expiration:
12 months from the date of shipment when stored properly.