Anti-PARK7 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q99497
Gene Id	11315
Gene Symbols	PARK7
Protein Name / Synonyms	PARK7, DJ-1, DJ1, GATD2, HEL-S-67p, protein DJ-1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

19kDa

Observed MW

20kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PARK7 antibody (144-00987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] PARK7 Polyclonal Antibody (144-00987)

Western blot analysis of extracts from normal (control) and PARK7 knockout (KO) 293T cells, using PARK7 antibody (144-00987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - [KO Validated] PARK7 Polyclonal Antibody (144-00987)

Immunofluorescence analysis of HeLa cells using PARK7 antibody (144-00987). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] PARK7 Polyclonal Antibody (144-00987)

Expiration:

12 months from the date of shipment when stored properly.

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